Nitric oxide (NO"¢) is an important signalling and defence molecule in higher eukaryotes, including plants and mammals. We recently showed that in A. nidulans this short-lived nitrogen oxide radical is generated during the nitrate assimilation process, and that detoxification by flavohemoglobin proteins FhbA and FhbB, which are co-regulated with the nitrate pathway, is required to protect nitrate- and nitrite reductase from nitrosative inactivation under elevated NO"¢ conditions (1). We here report the effect of nitric oxide donors and of mutations in fhbA and fhbB on developmental processes in two fungal genera. In response to external and internal signals, all fungi undergo developmental programs to form specialized structures and in A. nidulans, there is a fine balance between asexual (conidiation) and sexual development. We have found that addition of the NO"¢-releasing compound DetaNONOate reduced asexual development in A. nidulans. On the other hand, the formation of sexual structures is increased after DetaNONOate supplementation in several fungal species, including species from Aspergillus and Neurospora. (1) Schinko et al. (2010). Transcriptome analysis of nitrate assimilation in Aspergillus nidulans reveals connections to nitric oxide metabolism. Mol. Microbiol. 78: 720-738.
Full conference title:
26th Fungal Genetics Conference
- Fungal Genetics Conference 26th (2005)