NGS data revealed that the NSDA sterile mutant contains a mutation in the SCF ubiquitin ligase subunit gene, culC, in Aspergillus nidulans

DongSoon Oh, Dong-Min Han, Masayuki Machida, Kap-Hoon Han

Author address: 

Dept Pharmaceutical Engineering, Woosuk Univ, Wanju, Korea

Abstract: 

Sexual development and fruiting body production of fungi play pivotal roles in production of ascospores by meiosis as well as adaptation of various environmental changes. In a homothallic fungus Aspergillus nidulans, many environmental factors and genes affecting sexual development have been elucidated. One of the first and important attempts for understanding the sexual development of A. nidulans was isolation of NSD, BSD and ASD mutants, which are defective in the process. Among them, NSD mutants are divided into four different complementation groups, NSDA-D, and two of the mutants, NSDC and NSDD, have already been characterized about the responsible genes, nsdC and nsdDand their functions. However, nsdA and nsdBmutations are remained to be unveiled. Since classical complementation experiments were not successful, we analyzed the whole genome sequence of NSDA mutant obtained from Next Generation Sequencing (NGS) to identify the nsdA4mutation. As a result, we found three NSDA mutant-specific mutations and confirmed the mutations by PCR followed by sequencing analysis. One of the mutations was found in AN3939 locus which encodes SCF ubiquitin ligase subunit CulC. Themutation was G to T transversion, making D468Y amino acid residue change. Since the COP9 signalosome and ubiquitin ligase play important roles in fungal development, this mutation could be the correct nsdA4mutation responsible for the sterile NSDA mutant phenotype. However, since two more mutant-specific mutations were also found in NSDA, detailed genetic characterization and mutation analyses will have to be performed. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012R1A1A4A01012864).
2013

abstract No: 

233

Full conference title: 

27th Fungal Genetics Conference
    • Fungal Genetics Conference 27th (2013)