Loop-mediated Isothermal Amplification (LAMP) is the new gene amplification method developed by Eiken Chenical Co.Ltd (Tokyo, Japan) in 2001. Specific DNA amplification using LAMP needs four different primers designed from six targeted base sequences and reaction at a constant temperature (ca. 65C) using a strand displacement reaction for 15 to 60min. Result of amplification is visually observed by black light irradiation with premixed ethidium bromide. Using this new, specific, quick and inexpensive means, we developed molecular diagnostic system for deep seated fungal infections. Specific primer sets for Pan fungus, genus Aspergillus and genus Candida were designed in ribosomal RNA genes. All primer sets were specific to each target not only in vitro samples but also in vivo specimens. The successful result of the LAMP method for clinical specimens from candidosis and aspergillosis patents was also shown. The specific LAMP method will be a powerful tool for early diagnosis of life-threatening systemic mycoses and evaluation the efficacy of treatment.
Full conference title:
The 15 th Congress of the International Society for Human and Animal Mycology
- ISHAM 15th (2003)