Newer Antifungal Agents and Neutrophils (PMN) against Candida albicans (CA) Biofilms.

A. KATRAGKOU, A. CHATZIMOSCHOU, M. SIMITSOPOULOU, E. DIZA-MATAFTZI, C. TSANTALI, E. ROILIDES;

Author address: 

Aristotle Univ., Thessaloniki, Greece.

Abstract: 

Background: Biofilm (BF)-associated Candida infections are refractory to conventional antifungal agents. Little is known about the antifungal activity of host phagocytes and that of newer antifungal agents against CA BF. Our aim was to study the activities of 4 newer antifungal agents alone and in combination with PMN against CA BF and compare them to those against planktonic cells (PL). Methods: CA-M61, an intravascular catheter isolate, was used. PL were grown in YNB overnight, and BF on silicone elastomer disks in 96-well plates for 48 h at 37ºC. Voriconazole (VRC, 0.5, 2 or 32 mg/l), posaconazole (PSC, 0.25, 1 or 16 mg/l), caspofungin (CAS, 0.01 or 0.06 mg/l) or anidulafungin (AND, 0.12 or 0.5 mg/l) alone, PMN from healthy donors (E:T ratios 1:1 or 5:1) alone, or combinations of PMN and antifungals were incubated with BF and PL at 37ºC/5% CO2 for further 20 h. Damage of BF and PL was assessed by XTT metabolic assay. Analysis (n=7) included ANOVA and post hoc tests (p
2007

abstract No: 

M-1830

Full conference title: 

47th Interscience Conference on Antimicrobial agents and Chemotherapy
    • ICAAC 47th