A new in vitro model system to study antimycotic drugs

S. Cagnacci, A. Palenzona, E.A. Debbia, A. Marchese, R. Corví², L. Rossi

Author address: 

Genoa, San Remo, IT

Abstract: 

Objective: To help develop an in vitro bioassay to test antimycotic drugs that more closely reproduces the in vivo situation. To this purpose we are working on an experimental model consisting in the infection by Candida albicans of human epithelial and mesenchymal cells cultured in the 3-dimensional context of collagen gel. Methods: We used the oropharyngeal squamous carcinoma HSCO cell line established in our laboratory and the HECV endothelial cell line (ATCC) as a broad representative of mesenchymal cells. These cells were embedded in type I collagen gel. After the cells were grown the gels were infected with 1 x 104 particles/ml of Candida albicans, previously isolated from an immunocompromised patient and notable for being sensitive to amphotericin B (AmB) and resistant to ketoconazole (Kc). There were 3 experimental groups: (1) Candida alone; (2) Candida with HSCO cells and (3) Candida with HECV cells. After 24 hours from infection these cultures were treated with AmB and Kc at the concentration of 10 μg/ml. Candida particles were counted 24 hours later by the trypan blu-exclusion test. Results: We first evaluated the effects of the human cells on the survival of Candida. In the co-culture with HSCO cells the growth of Candida increased up to 100% over the control group. In the co-culture with HECV cells the proliferation of Candida was significantly inhibited (p
2006

abstract No: 

P736

Full conference title: 

16th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 16th (2006)