Purpose: To study prevalence and species distribution of medically important fungi in hospital indoor environment in Saint Petersburg. Methods: Air sampling was performed by means of impactor device PU-1B ("œHimko", Moscow). Sample volume was 250 L. Surfaces of walls, ceilings, floor, ventilation systems were swabbed and cultured on Petri dishes with Sabouraud dextrose agar. Results: During 2004-2008 7 hospitals (3 tertiary and 4 specialized) have been studied repeatedly for presence of fungi in the indoor environment. Totally, 1773 environmental samples, including 1239 air samples, have been investigated. Fungi have been cultured from 966 (77%) air samples and included 27 genera. Penicillium spp. were the most common moulds in hospital air (37,6% of culture positive samples) followed by Aspergillus spp. (29%). Species belonging to other fungal genera have been rarely isolated from hospital air. On the whole, 12 species of Aspergillus were found in the hospital air: A. fumigatus (15,1% of culture positive samples); A. niger (6,2%), A. versicolor (3,4%), A. flavus (1%) and other more rare species. The mean level of A. fumigatus spores in the air of operating rooms was 6-8 CFU per cubic metre while in the air of haematologic wards the highest concentration of this mould was 56 CFU per cubic metre. The sources of fungal contamination were determined to be sites of biodegradation on the walls, dust during reconstruction in the hospitals, contaminated ventilation systems. Conclusions: Wide range of fungal species, including opportunistic Aspergillus spp., were revealed in hospital air. Special measures should be implemented to minimize fungal contamination in hospital indoor environment.
Full conference title:
4th Advances Against Aspergillosis
- AAA 4th (2010)