Moulds in Hospital Air: Data from a Single Oncohematological Institution in Argentina

M.C. Dignani 1 *, D. Perrotta 2, R. Abrantes 2, L. Tufa 1, M. Brudny 2, N. Refojo 2, A. Hevia 2, M.C. Garcia-Damiano ~, E. Farias ~, G. Davel 2.

Author address: 

11FUNDALEU (Foundation ]:or the Fight Against Leukemia), 2Dpto. Micologfa, INEI, ANLIS Dr E.G. Malbrdn, Argentina


Background: Invasive mould infections (IMI) are a major cause of morbidity and mortality among patients with hematoLogicaL malignancies. Different institutions have different epidemioLogy of IMI. Hospital air can be a reservoir for moulds. In our institution, Aspergillus spp. are the most frequent cause of IMI foLLowed by Alternaria spp. Objectives: We conducted a one-year prospective study to evaluate the epidemioLogy of moulds in our hospital air. Methods: The air of 44 indoor (16 rooms [4 waLLHEM, 6 portable HEPA], 13 bathrooms, 15 common areas) and 5 outdoor areas were tested 3 times a day (morning, noon, afternoon) once a month, four months a year (spring 2004, summer, faLL, and winter 2005) using a portable volumetric air sampler (SAS SUPER 90, pbi InternationaL, MiLano, ItaLy). MaLt extract agar added with cLoramphenico[ (250mg/[) was used as medium culture. PLates were incubated for 3-5 days at 25°C. CoLony counts were done by conventional methods. MouLds identification was made by culturing them on potato dextrose agar. CoLony counts were enumerated as coLony-forming units per m 3 of air (CFU/m3). Results: 660 indoor and 75 outdoor air samples were obtained. The airborne concentrations of moulds are shown in the table. The distribution of genera is shown in the figure. The most frequent opportunistic fungi found in indoor and outdoor air were Aspergillus spp. fob towed by Alternaria and Fusarium spp. Conclusions: We found a high toad of moulds in hospital air with a median that ranged from 78 to 263 CFU/m 3. The highest counts were observed in summer. The most prevalent genera in indoor air were Aspergillus foLLowed by Alternaria and Fusarium spp. This distribution of genera matches our Local epidemioLogy of IMI.

abstract No: 


Full conference title: 

14th International Symposium of Infections in the Immunocompromised Host
    • ISIIH, 14th