Morphological, biochemical and molecular identification and epidemiological investigation of Candida species in Yasuj

F. Alizadeh 1, A. Khodavandi 2, M. Jahanbazi 1, P.P. Chong 3

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Background: Clearly, Candida is able to cause wide spectrum of diseases including cutaneous, oral or systemic candidiasis. During the past two decades, Candida was observed to be the fourth most common agent (5-10%) of nosocomial blood-stream infections. Recently, disseminated candidiasis was found to be high mortality in excess of 25% even with current antifungal therapy. New findings also showed that Candida infections are not only limited to neutropenic or immunocompromised patients but all types of patients also could be involved. In the present study, Candida species was isolated and identified from humans using different methods. Materials and methods: The distribution of Candida species was examined using 226 yeasts from various clinical specimens including control, patients and persons with underlying disease in yasuj during 12 months. The isolates were identified by germ tube test, hyphal/pseudohyphae, chlamydoconidia production, carbohydrate assimilation and fermentation test, nitrate assimilation and urea hydrolysis. Eventually, DNA of isolated yeasts was extracted and amplified using ITS1 and ITS4 universal fungal primers and then sequenced. The sequence similarity was analyzed using nucleotide Blast software against the nonredundant GenBank database in the NCBI website and confirmed. Results: Among the 226 isolates studied, 3 species of Candida were identified and the relative frequency of isolation was C. albicans (89.4%), C. tropicalis (6.2%), C. guillirmondi (0.9%) and unknown species (3.5%). The isolate from vaginal swabs was predominantly C. albicans (83.3%), C. tropicalis (8.3%), C. guillirmondi (5.6%) and unknown species (2.8%). While, Non-C. albicans was the most common Candida species isolated from blood and skin. Conclusion: These favorable results demonstrate that C. albicans is still the most predominant species in normal, patients and persons with underlying disease. On the other hand, there is not any significant correlation between case study factors and variety of Candida species. Keywords: C. albicans, C. tropicalis, C. guillirmondi, candidiasis.

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Iranian Congress on Medical Mycology
    • ICMM 2011