Miller DR, Demi SM, Roberts GD

Author address: 



To improve communication in different areas of our clinical mycology laboratory, we developed a Web-based system to track the quality assurance of fungal smears and yeast antifungal susceptibility testing. Databases for each test type were developed using Microsoft ® Access 97 SR-2 Database Management (Access) for data collection and analysis of quality control, proficiency and test results. Daily positive and negative control and test results are entered at computers at each workstation on a dedicated Web-based server. Antifungal susceptibility results, performed with the YeastOne ® , Sensititre, [Trek Diagnostic Systems, Inc. Westlake OH] for Amphotericin B, Fluconazole, Itraconazole, Ketoconazole and 5-Flucytosine were entered into Access and then exported to Microsoft ® Excel 97 SR-2 to produce line graphs. We have analyzed quality control data from January 1, 2002 to December 31, 2002 for C. krusei (ATCC 6250) n=144 and C. parapsilosis (ATCC 22019) n=143 and 844 clinical strains that represented 20 yeast species of Candida, Cryptococcus, Rhodotorula, Saccharomyces and Trichosporon. There were two quality control failures. Quality control of fungal smears was also monitored using Access. During this same time period we performed 218 positive and negative controls for Calcofluor/KOH with no failures, and 217 Calcofluor (Fungi-Fluor™, Polysciences Inc., Warrington PA) for Pneumocystis, with one failure. All failures of all quality control testing are entered into an Access event management database that is reviewed at the division level. This Web-based system provides better compliance of recording data since it is more accessible, provides consistent multi-user data entry, makes data analysis easier, and trending analysis can be done. Data can be entered from any workstation including the BioSafety Level III suite if necessary. Event management can be reviewed and audited at different workstations. This system has also made quality assurance data more accessible and easier to interpret by accreditation agencies such as College of American Physicians. Other databases for other quality assurance issues in the clinical mycology laboratory are under development.

abstract No: 


Full conference title: 

The 15 th Congress of the International Society for Human and Animal Mycology
    • ISHAM 15th (2003)