Molecular investigation of Aspergillus infections in an Iranian training hospital using RAPD-PCR

Atefeh Namaki


Background: Aspergillosis is known to  be an airborne  infection and the nosocomial infections are associated with constructions  and  increased  dust  loads  in hospital  indoors. Our main object  was to find the environmental sources of Aspergillus species causing hospital acquired infections.

Material/methods: The clinical and environmental samplings were performed during 18 months from spring 2010  to summer 2011 in a  large  educational hospital. A  morphological  diagnosis was used including media culture, for  the first identification of  all isolated Aspergillus species. For the RAPD assay, extraction of DNA was performed using manual phenol-chloroform method followed  by PCRwith six random primers. The results of RAPD were compared between the clinical Aspergillus isolates and hospital indoor isolates.

Results: Use of RAPD method resulted various differential patterns so that some Aspergillus isolatesfrom the clinical and hospital indoor including the pairs 32 and 45 among 51 Aspergillus pairs, werecompletely matched. Some other Aspergillus pairs: 16, 31 and 237 were not matched. The Aspergillus isolate of pair 32 was obtained from both bronco alveolar lavage and air conditioner as a same RAPDtype of A. niger.  Also, the same RAPD type of A. flavuswas isolated from sinus discharge as well as the walls surface.

Conclusions: The hospital  sources for the Aspergillus  clinical isolates  included  air  conditioners and rooms walls  and RAPD-PCR analysis can play a  trivial role  in finding the hospital  sources  of Aspergillus clinical isolates. 


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European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 26th (2016)