Molecular identification of Trichosporon dermatis as a cause of fungemia in a pediatric patient undergoing a combined liver and small bowel transplant

Iwen1, P.C.A., Noel2, R.K., Sudan1, D.L., Freifeld1, A.G.

Author address: 

1 University Nebraska Medical Center, OMAHA, NEBRASKA, USA 2University Nebraska Medical Center, OMAHA, NEBRASKA, USA

Abstract: 

The first reported case of fungemia caused by Trichosporon dermatis in a solid organ transplant patient is described. The patient was a 14-month old female born prematurely who developed a short gut syndrome secondary to jejunal necrotizing enterocolitis with subsequent TPN-induced cholestasis leading to a combined liver and small bowel transplant. The organism, which was recently moved from the Cryptococcus genus to Trichosporon, was isolated from multiple blood cultures and originally identified as a Cryptococcus humicola using the API 20C (BioMerieux, Durham, NC) and the Vitek yeast identification systems (bioMerieux). Molecular testing using comparative sequence analysis with the internal transcribed spacer (ITS) regions within the rDNA locus as targets subsequently identified the isolate with a 99.9% similarity as either a T. mucoides or T. dermatis. Species verification was performed using the intergenic spacer (IGS) 1 region within the rDNA locus as the target which confirmed the isolate as T. dermatis with a 99.9% sequence similarity. This case showed the utility of using the IGS-1 molecular target for the identification of this rare pathogen and extends the conditions associated with invasive disease caused by T. dermatis. Future studies are needed to validate these and other molecular targets for their application to optimal patient management.
2007

abstract No: 

P196

Full conference title: 

3rd Trends in Medical Mycology
    • TIMM 3rd (2011)