Molecular identification of candida species isolated from dialysis and renal recipient patients

M.Shalchian 1, A.Sabokbar 1, M.Riazipour 2, M.Saghazadeh 3, SH.Yasaee 4

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Background: Infection remains the most common cause of morbidity and mortality after organ transplantation .In other hand patients on hemodialysis have limited urinary excretion that makes them susceptible to infectious diseases that yeast are well-known problems in them .There are 10-15 pathogenic Candida species. Although Candida albicans is the most prevalent and important causative agent of Candida infection, the important of C. parapsilosis, C. tropicalis, C. krusei, C.glabrata, C. guillermondii have increase significantly as they tend to be more resistant to antifungal agents. Therefore, timely diagnosis of Candida species in the absence of infection rates still are low level and the beginning of antifungal therapy effective in inhibiting the survival of patients with immune system is very helpful. Materials and Methods: This study identified Candida species isolated from 48 samples of dialysis and renal recipient patients that was tested and identified by direct observation and culture on Corn Meal Agar and Sabouradus Dextrose Agar, the genomic DNA using method glass bead / phenol chloroform was isolated and PCR-amplified using two primer pairs [primer P4501 (base pairs1021 to 1043), primer P4502 (base pairs 1370 to 1351)]. The PCR products were digested with two restriction enzymes HincII, NsiI separately. Results: Analysis of PCR product, and using HincII, NsiI as restriction enzyme, allow us to identify two medically important fungi, 37(82%) Candida albicans and 8(17%) Candida guillermondei, according to different size band in polyacrylamide gel electerophoresis. Conclusion: It seems that this panel of PCR-RFLP coud be a rapid and useful molecular approach in diagnostic studies of invasive opportunistic fungal infection. Keywords: Hemodialysis, Candida albicans, restriction enzymes.

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Iranian Congress on Medical Mycology
    • ICMM 2011