Molecular identification and aenotyping of Trichosporon asahii isolated from Indian patients

Prasanna Honnavar1*. Shivaprakash M Rudramurhy1, Vijayalatha Rastogi2, Anup Ghosh1, Joseph Jillwin1, Arunaloke Chakrabarti1

Author address: 

1Mycology Division, Dept, of Medical Microbiology, PGIMER, Chandigarh-160 012, 2' Dept, of Microbiology, J.L.N Medical College & associated group of Hospitals, Ajmer-305 001

Abstract: 

Trichosporonosis due to Trichosporon asahii is a life-threatening infection with a very poor prognosis. Although Trichosporonosis is emerging mycoses, the T. asahii genotypes prevalent in Indian patients are not known. We analyzed the sequence ofITS-5.8S and intergenic transcribed spacer (IGS) region 1 oftherDNAlocusin25 T. asahii, isolated from different parts of India. The sequences were compared with GenBank databases. The sequences were aligned using ClustalX2. The phylogenetic tree of clinical isolates along with different genotypes was constructed by Neighbor-Joining method. The distances between sequences were calculated using Maximum Composite Likelihood model. ITS-5.8S region failed to differentiate among different genotypes. Of the nine identified T. asahii genotypes, isolates that originated in India were of genotype 3 (40%), followed by 1 (28%) and 7 (28%). Genotype 1 is the most abundant type of T. asahii strain in all countries, except for the United States (genotype 3 and 5). Genotype 7 has been found only in Japan and that genotype 8 and 9 were described only in Turkey. ITS region sequencing is unsuitable for identification and genotyping of T.asahii isolates. Comparative sequence analysis suggested that the divergence of IGS 1 sequences has been greater than that of the ITS regions. In contrast to studies from Turkey, Thailand and Japan, our result shows that genotype 3 is predominant in India as in USA. Our study confirms presence of genotype 7 in India like Japan. In addition to the nine known T. asahii genotypes, one novel genotype was identified. 9830;Corresponding author E-mail:[email protected]
2014

abstract No: 

OP-013

Full conference title: 

Society for Indian Human and Animal Mycologists 2014
    • SIHAM