Micafungin Enhances Neutrophil Oxidative Burst against Candida and aspergillus.

C. GIL LAMAIGNERE, F.M.C. MÜLLER

Author address: 

Dept. of Pediatrics, Univ. Heidelberg, Heidelberg, Germany.

Abstract: 

Background: Micafungin (MCF) is a member of the candin family with activity against Candida and aspergillus spp., that has been shown to inhibit adherence of C. albicans to epithelial cells. Methods: Neutrophils (PMN) were purified from healthy donors by dextran sedimentation / ficoll centrifugation. After hypotonic lysis of erythrocytes, PMN were resuspended in Hank's balanced salt solution. Blastospores of C. albicans ATCC #90028 and hyphae of A. fumigatus #4215 were opsonized with 50% human serum. The oxidative burst of PMN at 106 /ml in absence (unstimulated) or presence of 1µM FMLP, 106 blastoconidia/ml (C. albicans) or 106 hyphae/ml (A. fumigatus) was evaluated by a cytochrome C colorimetric method. (N=6). Statistical differences were evaluated by paired ANOVA with Dunnet's correction or with post-test for linear trends. Results: The average nM O2- of 6 experiments produced by PMN in 30 min are depicted in the table. * indicates significant difference from unstimulated PMN evaluated by ANOVA with Dunnett correction (P
2003

abstract No: 

M-383

Full conference title: 

43rd Interscience Conference on Antimicrobial Agents
    • ICAAC 43rd