Isolation and characterization of nsdD suppressor mutants in Aspergillus nidulans.

Jee Hyun Kim, Dong Beom Lee, Jung Youl Min, Kap-Hoon Han, Kwang-Yeop Jahng, Dong-Min Han.


The nsdD gene encodes a GATA type transcription factor, carrying a type IVb zinc finger DNA binding domain, which functions in activating sexual development of A. nidulans. A number of suppressor mutants of nsdD were isolated and characterized. According to the phenotypes of growth rate and developmental pattern, nine mutants were selected and recombinants carrying suppressor mutation but free of nsdD mutation were isolated through genetic crosses with wild type. Most of suppressor mutants developed plenty of cleistothecia even under the conditions, such as high osmolarity(e.g.1 M KCl), visible light or acetate as a sole C source, where sexual development of wild type is completely inhibited, implying that the genes might be involved in the regulation of sexual development in response to the presence of those environmental factors. All mutant alleles were recessive to that of wild type. They were grouped into five complementation groups and the respective genes were designated as sndA, sndB, sndC, sndD and sndE. ThesndA and sndC were linked to linkage group II, sndB and sndD to linkage group V and sndE to linkage group VII. Some of those genes includingsndB were cloned and sequence-analyzed.

abstract No: 

Fungal Genet. Newsl. 50 (Supl):abstract

Full conference title: 

22nd Fungal Genetics Conference
    • Fungal Genetics Conference 22nd (2001)