Heterotrimeric G proteins, consisting of alpha, beta and gamma subunits, are implicated in major signal transduction pathways governing the various cellular functions in eukaryotic organism. To study the role of G protein in differentiation of the filamentous fungus A. nidulans, we isolated ganA gene encoding Galpha protein homolog from A. nidulans by PCR amplification using degenerate oligonucleotide primer and analyzed its nucleotide sequence. Open reading frame of ganA containing 361 amino acids is highly related to alpha-subunits of heterotrimeric G proteins in other organisms. DNA fragment containing ganA cDNA could partially complement gpal point mutation of S. cerevisiae. By targeted integration we made deletion mutations of ganA in which the internal fragment was replaced by the argB gene. We also constructed dominant-activating mutation and overexpression clone of ganA. However these mutations showed no significant phenotypes in asexual or sexual development. From these mutants, we are determining the cellular function of ganA in development and growth. This work was supported by grants for Research for Genetic Engineering from KRF.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)