Invasive aspergillosis caused by Aspergillus ustus, case report and review of the literature

P. Verweij, M. van den Bergh, P. Rath, B. de pauw, A. Voss,J. Meis.


Objectives: To describe invasive aspergillosis by Aspergillus ustus in a bone marrow transplant (BMT) recipient and compare it with those described in the literature and to determine the in vitro activity of amphotericin B (AB), itraconazole (ITZ), voriconazole (VCZ) and terbinafine (TB) against 12 clinical and environmental A.ustus isolates.Methods: The MIC was determined by a broth microdilution method according to the proposed standardized procedure for antifungal susceptibility testing by the NCCLS. Each drug was two-fold diluted to obtain concentrations ranging from 64 to 0.06 mg/L. The plates were incubated at 37'C and read after 24 and 48 h of incubation. For AB, the MIC endpoint was defined as complete inhibition of visible growth, and for TB and the azoles, 75% growth inhibition compared with control wells. Subcultures from the wells with the lowest drug concentrations showing 99.9% reduction from the original inoculum size were judged to contain the minimal fungicidal concentration (MFC). The literature for the years 1960-98 was reviewed for case reports of invasive aspergillosis caused by A. ustus.Results:

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9th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 9th (1999)