The A. nidulans early- and late-Golgi is formed by a dynamic network of rings, tubules and fenestrated structures that is strongly polarised. Early and late Golgi cisternae are optically resolvable, allowing multidimensional imaging studies on cisternal maturation. Amongst the many factors that contribute to maintain the identity and structure of the Golgi is Rab6 (RabC in A. nidulans) that, unexpectedly, also appears to play a role in the Spitzenkörper. rabC) mutants display a marked impairment in apical extension but its most remarkable phenotype is that they have conspicuously fragmented and brefeldin A-insensitive early and late GEs, indicating that RabC contributes to the organisation of the Golgi. As in yeast RacC is involved in the recycling from endosomes to the Golgi of the vacuolar hydrolase receptor VpsTVps10, but not in the traffic of the synaptobrevin homologue SynA between endosomes and the PM, tlg2) mutants grow normally but are synthetically lethal with rabC), indicating that RabC plays Tlg2-independent roles. Our data underscore how wanting our understanding of the complexities of membrane traffic in filamentous fungi is, in spite of its major economic impact in Biotechnology and Medicine.
Full conference title:
26th Fungal Genetics Conference
- Fungal Genetics Conference 26th (2005)