The aim of this study was to identify antifungal lactic acid bacteria and characterize their activity against the dermatophyte Microsporum and Epidermophyton. 220 different lactic acid bacteria were isolated and initially screened for anti- Aspergillus activity. Four strains, which exhibited strong inhibitory activity were then tested against the dermatophytes Microsporum canis, Microsporum gypseum and Epidermophyton floccosum which they also caused inhibition as observed by large fungal clearing on agar surface. The strongest inhibition was seen with Lactobacillus reuteri ee1p. When freeze-dried cell-free supernatant powder from this strain was incorporated in culture medium at concentrations greater than 2%, fungal colony growth was inhibited. Addition of the powder at 5% to liquid cultures caused complete inhibition of fungal growth on the basis of turbidity. Reuterin was undetectable in all four antifungal strains. The cell free supernatant of Lb. reuteri ee1p was run through the HPLC. The spectrum produced was compared to a standard chromatoGram-of known anti-fungal compounds to help identify the agents responsible and several possible candidates were identified.
Full conference title:
Society for General Microbiology
- SGM 2012