INFLUENCE OF CULTURE MEDIA ON THE SUSCEPTIBILITY OF CANDIDA SPECIES TO FLUCYTOSINE (5FC), AMPHOTERICIN (AMB), FLUCONAZOLE (FLC), VORICONAZOLE (VOR), AND ITRACONAZOLE (ITR)

Fegeler W 1 , Becker K 1 , Schmalreck A 2 , Members of the Working Group Clinical Mycology

Author address: 

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Abstract: 

Purpose. Since different culture media were recommended by the protocols for antifungal susceptibility testing using microdilution test (NCCLS M27A, DIN 58940), the influence of three different media on MIC values to five systemic antifungal agents was evaluated during the course of a German multicenter study comprising 16 centers. Methods: For a total of 420 clinical Candida isolates (C. albicans, 49.5%), the MICs were determined by a microdilution assay for three media according to DIN 58940. All media (RPMI1640; HR medium; HR with 0.25 mg/L methylen blue, HRM) were used with a final concentration of 2% glucose. With respect to their susceptibility to the different agents, isolates were stratified in three categories: susceptibility class (SC) A, B and C with SC limits for 5FC, FLC and ITR according to NCCLS M27A. For VOR and AmB the tentative class limits were defined: SC-A MIC •0.25 mg/l (VOR and AmB); SC-B MIC > 0.25 - 0.25 -
2003

abstract No: 

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Full conference title: 

The 15 th Congress of the International Society for Human and Animal Mycology
    • ISHAM 15th (2003)