Many phytopathogenic fungi have both teleomorph and anamorph, and differentiate from hyphae depending on the conditions in the host plants. The fungi often lose the morphogenic ability and grow only in the hyphae form by the succession of in vitro culture. They, however, recover the ability if the hyphae could infect to the host plants. We have developed the method (ALP medium) that many fungi could form perithecia in vitro using lightly boiled gardenia and hydrangea leaves (Furukawa and Kishi 2000). These facts indicate that some common factors which induce perithecia formation in many fungi must exist in the leaves. We tried to extract the factors and re8208; investigate physical conditions, especially light wavelength, to induce perithecia. Pleospora herbarum (Pers.) Rabenh was used for the examinations. As a result, the activity existed in the oxidized aqueous fraction, which induced the perithecia and conidia abundantly under the dark condition. Fatty acids and the related compounds, which were reported to induce perithecia and conidia in Aspergillus nidulans (Calvo et al. 1999, Tsitsigiannis et al 2004, 2007), did not induce perithecia or conidia under light or dark conditions. Blue light, which is essential for conidia formation in Neurospora crassa (Olmendo et al. 2010), was not needed for P. herbarum on the medium containing oxidized leaf extract, though it was essential for perithecia production on other media. Those imply the morphogenic switch of phytopathogenic fungi is different from that of saprozonic fungi.
Full conference title:
11 th European Conference on Fungal Genetics
- ECFG 11th (2012)