Inducible expression of Aspergillus nidulans uvsJ by mutagenic DNA-damage and specific accumulation of UvsJ protein in conidial nuclei.

Su Jin Cho, Suhn Kee Chae and Pil Jae Maeng.

Abstract: 

Aspergillus nidulans uvsJ gene is known to encode a ubiquitin-conjugation enzyme and to be included in UvsF group, one of the four epistatic group of DNA repair genes. The presumptive functional analogue of uvsJ, RAD6, has been reported to be involved in various cellular processes, such as sporulation, DNA repair, ubiquitination, cell cycle control, in Saccharomyces cerevisiae. In the present study, we analyzed the expression ofuvsJ and the localization of UvsJ protein using dimeric sGFP as a vital reporter. Under normal conditions, only a little transcription of uvsJ was detected through the whole thallus when monitored in both uvsJ-p::(sgfp)2 anduvsJ::(sgfp)2 transformants. On the other hand, when the cells of the transformants were subject to mutagenic DNA-damage rendered by alkylating chemicals (MMS, EMS, and 4-NQO), UV, and heat shock, the level of uvsJ expression was increased both in vegetative and asexually differentiated cells. Quite surprisingly, UvsJ::(sGFP)2 protein was specifically accumulated in the nuclei of conidiospores under the conditions of mutagenic DNA-damage. These results suggest that the expression of uvsJ is controlled at both transcriptional and post-transcriptional levels.
2003

abstract No: 

Fungal Genet. Newsl. 50 (Supl):abstract

Full conference title: 

22nd Fungal Genetics Conference
    • Fungal Genetics Conference 22nd