Increased Sensitivity to ,L-4 in Cystic Fibrosis Patients with Allergic Bronchopulmonary Aspergillosis

J. Smick j, M. Warrier l, V. P. Kurup 2, P. S. Hutchinson ~, A. P. Knutseni

Author address: 

J Allergy/lmmunology, St. Louis University, St. Louis, MO. 2Allergy/Immunology, Medical College of Wisconsin, Milwaukee, WI.


RATIONALE: ABPA is characterized by a heightened Th2 CD4+ T-cell response to A. fumigatus allergens and a hyper-lgE state compared to CF patients without ABPA. We hypothesize that one reason for this response is increased sensitivity to IL-4 in ABPA resulting in increased expression of CD23 and CD86 and leading to a positive amplification mechanism that increases Tb2 CD4+ T cell responses. METHODS: PBL were stimulated with rlL-4 (range 0.1-10 ng/ml) and rIL-13 (range 1-10 ng/ml) for 48 hours. The number of CD23 molecules and percentages of CD23+ B cells were quantified by flow cytometry. RESULTS: The percentages of CD23+ and CD23+CD86+ B-cells were significantly elevated in vivo in ABPA CF patients compared to both non- ABPA CF patients and non-atopic controls. Following IL-4 stimulation in vitro, the numbers and percentages of CD23 expression on B-cells and CD86+ B-cells were significantly up-regulated in ABPA CF patients compared to non-ABPA CF patients and controls. The slope of increase of CD23 molecules on B-cell was significantly increased in ABPA patients compared tom non-ABPA patients. With IL-13 stimulation, CD23 expression was up-regulated; however, there was no significant difference in ABPA CF patients compared to non-ABPA CF patients and controls. CONCLUSIONS: Thus, ABPA CF patients have increased expression of CD23+ and CD23+CD86+ B-cells and increased sensitivity to IL-4 stimulation but not to IL-13 stimulation with up-regulation of CD23 molecules compared to non-ABPA CF patients. Funding: Cystic Fibrosis Foundation

abstract No: 


Full conference title: 

2003 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2003 (59th)