RATIONALE: We have previously demonstrated that susceptibility to developing allergic bronchopulmonary aspergillosis (ABPA) is associated with certain HLA-DR2 alleles. Inserting the susceptibility allele, HLA-DRβ 1 1503, into transgenic mice followed by sensitization and challenge with Aspergillus fumigatus (Af) resulted in an allergic inflammation in the lung consistent with ABPA. Insertion of a non-susceptibility allele, HLA-DRβ 11502, resulted in a non-allergic inflammatory response. The pulmonary surfactant protein SP-D is thought to be an important modulator of immune and inflammatory responses in the lung. We hypothesized that SP-D levels in BAL would be elevated in our transgenic mice who demonstrated the allergic inflammatory response. METHODS: Transgenic strains of mice were developed which expressed either the susceptibility allele 1503 or the non-susceptible allele 1502. All mice underwent both intraperitoneal and nasal sensitization with Af. BAL was performed at days 7 and 14 after tracheal challenge with Af. BALF was collected and assayed for SP-D by Western blot and ELISA. RESULTS: Mice which expressed the susceptibility allele for ABPA, 1503, showed a greater increase of SP-D in BAL at both 7 and 14 days following Af challenge than mice which expressed 1502. CONCLUSIONS: Elevation of SP-D in BAL is seen in transgenic mice who express HLA-DRβ 11503 and develop an ABPA-like picture of allergic inflammation in response to challenge with Af. This is probably associated with the known effect of SP-D as a modulator of immune and inflammatory responses.
Full conference title:
2006 American Academy of Allergy, Asthma, and Immunology Annual Meeting
- AAAAI 2006 (62nd)