The aim of this study was to determine the prevalence of E. coli O157:H7 in pediatric patients with acute gastroenteritis. A prospective epidemiological study was performed in cases and controls to detect the germ in fecal samples of 649 children (0-7-year-old) with acute gastroenteritis, in Ankara, Turkey. The stools were inoculated to Sorbitol MacConkey agar (SMAC). The sorbitol negative colonies were biotyped and seroagglutinated with a specific antisera (Difco, USA) for E. coli 0157:H7 and also researched the production of verotoxins in Vero cell culture. Of the 649 stool specimens 23 (3.54%) sorbitol negative E. coli strains showed the presence of low titer agglutination with 0157:H7 antiserum. However, these strains were negative for verotoxin production. False-presumptive-positive results were observed with strains of E. coli when isolated by the culture method and when using a commercially purchased antisera. This false presumptive positive results can be reduced by detection of verotoxin production at these strains. As a result of this study we believe, that we are in a low rate of infection area for E. coli O157:H7 and also accordingly do not recommend routine screening of this pathogen by only using Sorbitol MacConkey agar and antisera.
Full conference title:
11th European Congress of Clinical Microbiology and Infectious Diseases
- ECCMID 11th (2001)