INTRODUCTION: The controlled explosion of the maternity hospital of our institution, provided us with a unique opportunity to study the load of filamentous fungi outside and inside the hospital before, during and after the demolition. MATERIAL AND METHODS: External (5 different points) and internal air (protected and non-protected areas) were sampled by means of an automatic volumetric machine (MAS- 1 00 Air Sampler). Each sample was taken twice to analyze the reproducibility of our system. Samples were taken at least daily during the week before the demolition (basal counts), at minutes 10, 30, 60,90, 120, 180, 240, 420, 540, 660 the demolition day, daily during the week after demolition and weekly during weeks 2 and 3 after demolition. Plates (Sabouraud glucose irradiated) were incubated at 37`C for a period of 5 days and read daily. Clinical follow~up was also performed. RESULTS: Overall, 352 samples were obtained (150 external, 82 from protected internal zones and 120 from non-protected internal areas). Median spore counts of the external air raised from 16 cfu/m3 to 70 cfu/m3 on the demolition day. They returned to previous levels on day 11. Inside the hospital, non protected areas raised from 7 cfu/m3 to 36 cfu/m3 on the demolition day. They returned to levels between days 12-18. Most protected areas (oncology, operating rooms) showed no spores before the demolition day and remained negative the demolition day, showing the efficacy of the HEPA filters. No significant differences were found between the two sets of samples taken each time (Intraassay variance: 2.4 cfu/m3). No episodes of invasive filamentous mycosis have been detected to date after the demolition day.CONCLUSIONS: Our data show a clear elevation of count of filamentous fungal spores link to a demolition by controlled explosion. This claims for the adoption of special protected measures in such situations.
Full conference title:
5th Trends in Invasive and Fungal Infections
- TIFI 5th