IMMUNE RESPONSES INDUCED BY HEAT KILLED YEAST, A VACCINE AGAINST ASPERGILLOSIS IN MICE

M. Liu1,2,3*, K. Clemons1,2,3, M. Bigos2, D. Stevens1,2,3

Author address: 

1California Institute for Medical Research, San Jose, CA 2Stanford University, Stanford, CA 3Santa Clara Valley Medical Center, San Jose, CA

Abstract: 

Purpose: Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. To elucidate the underlying mechanism of protection we explored the immune responses induced by HKY. Methods: BALB/c mice (5-week) were given 107.8 HKY subcutaneously 1/wk for 3 wks. Bronchoalveolar lavage (BAL) fluid, spleen or lymph nodes, and serum were collected 2-5 weeks later. Spleen or lymph node cells were cultured (105 cells/well) at 370C overnight before stimulation with HKY. Cell proliferation was tested by Alamar blue reduction 3d (spleen) or 7d (lymph node), and cytokines measured by ELISA 1d (spleen) or 5d (lymph node), after stimulation. Antibody to glycans was tested by ELISA. Four weeks after vaccination, spleen mononuclear cells were stimulated with HKY (64 h), stained for specific cell markers and with carboxyfluorescein diacetate succinimidyl ester, and analyzed by flow cytometry for proliferation of lymphocyte populations. Results: In spleen cells of HKY-vaccinated, compared to non-stimulated controls, 103.6 and 105.6 HKY promoted proliferation (all P
2010

abstract No: 

20

Full conference title: 

4th Advances Against Aspergillosis
    • AAA 4th (2010)