Imaging actin dynamics in Aspergillus nidulans using Lifeact.

Brian D. Shaw, Laura Quintanilla, Srijana Upadhyay, and Zaida Hager Dept.

Author address: 

Plant Pathology and Microbiology, Program for the Biology of Filamentous Fungi, Texas A&M University, College Station, TX 77843-2132, USA


Polarization of actin to the hyphal apex is essential for hyphal growth. Previous work in A. nidulans has shown a sub-apical collar of actin::GFP patches that is associated with endocytosis and is necessary for growth. Here we use the Lifeact construct, an actin binding domain fused to either GFP or RFP, to image both actin patches and cables during growth. In addition to the sub-apical collar of patches in growing cells, we also note actin cables organized to the Spitzenkorper in growing tips. We also report here a new structure that we term the Sub-apical Actin Web (SAW). The SAW can be described as a complex array of actin cables distal to the tip in growing cells. This array is stable on the distal face but is highly dynamic on the proximal face with cables bending, retracting and growing toward the apex. Results of co-localization studies will be discussed.

abstract No: 


Full conference title: 

26th Fungal Genetics Conference
    • Fungal Genetics Conference 26th (2005)