IgE Response to Recombinant Allergens in Patients Sensitized to Aspergillus in Asthmatics and Cystic Fibrosis

u P. Kurup I , A. P. Knutsen 2, P. A. Greenberger 3, L. J. Castillo I , N. Elms i , M. B. Levy I, K. J. Kelly I, J. N. Finkl; IPediatrics

Author address: 

Medical College of Wisconsin, Milwaukee, WI, 2Pediatrics, St. Louis University, St. Louis, MO, 3Medicine, Northwestern University School of Medicine, Chicago, IL


RATIONALE: Aspergillus fumigatus (Af) induced allergic diseases such as ABPA have been reported in asthmatics and patients with cystic fibrosis (CF). Early diagnosis is essential for better patient care and control of the disease. Demonstration of specific IgE antibody is considered as significant criterion in the diagnosis of ABPA. Currently, a number of major allergens from Af have been cloned, expressed, and purified. Based on recent findings, we hypothesized that recombinant (r) allergens can be useful in the diagnosis of ABPA, and hence we evaluated different groups of patients using rallergens. METHODS: We studied normals, patients with ABPA, CF, and CF with ABPA or Af sensitization alone. The diagnostic criteria were those recommended by the Cystic Fibrosis Foundation. ImmunoCAP and ELISA with recombinant Asp f 1, f 2, f 3, f 4, and f 6 were carried out to detect specific IgE antibodies in the sera of the subjects. RESULTS: The results demonstrated that normals and CF alone were negative and Asp f 2 was most effective in detecting specific lgE in ABPA (87%), while Asp f 4 was most effective in CF (85%) with Af sensitization. ABPA showed markedly increased lgE antibodies to all 5 allergens studied. CF with or without ABPA, but with Af sensitization reacted predominantly to Asp f 4. CONCLUSIONS: The results indicate that ABPA patients have significant levels of IgE to recombinant allergens, but atopic CF with skin reactivity to Af cannot be differentiated from CF with ABPA. Funding: AAAAI; VA Medical Research

abstract No: 


Full conference title: 

2003 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2003 (59th)