IDENTIFICATION OF YEASTS OF CANDIDA SPECIES FROM PATIENTS WITH VULVOVAGINITIS IN THE AREA OF CARACAS, VENEZUELA

1 de la Parte MA, 2 Brito A,Mariño C 3 Mendoza M, Alvarado P

Author address: 

1 Nursing School, Faculty of Medicine, Central University of Venezuela (UCV), Caracas, Venezuela 2 JM Vargas Medical School, Faculty of Medicine, UCV, Caracas, Venezuela 3 Mycology Laboratory, Institute of Biomedicine, Caracas, Venezuela.

Abstract: 

Vaginal candidiasis is a fungal infection distributed around the world, produced by yeasts of the Candida species belonging to the vagina normal flora. Yeasts of the Candida species are found in 20 to 30% of all the cases of vulvovaginitis and Candida albicans is responsible of the great majority of cases. Notwithstanding, more recent studies show an increase of other Candida species producing this pathology. Some "œno albicans" Candida species have the particularity to show resistance to the antifungal agents used for this pathology and for this reason, we should determine accurately the species involved in each case, to install the specific treatment. The objective of our study was to identify the species of Candida yeasts associated to cases of vulvovaginitis in patients from the metropolitan area of Caracas during the year 2003. We studied 35 samples obtained from the vaginal fornices and walls; each sample was examined on fresh smear treated with 10% KOH and cultivated in Sabouraud-antibiotic medium. In order to determine the species, the following assays were performed: germinal tube production and clamydospore formation in wheat flour-Tween 80 broth, observation of colony color in CHROMagar Candida, morphology in corn-meal and assimilation of sugars. We used controls for C. albicans, C. tropicalis and C. glabrata. Among the Candida species, C. albicans was the most frequently found (88,6%: n=31); C. tropicalis represented 5,7% (n=2) and C. glabrata 5,7% (n=2). Our results are similar to those found by other groups in Venezuela reporting frequencies for C. albicans between 50 and 87,5% of their series and internationally, C. albicans is always the most common species isolated. This group of vaginal samples are part of a larger project being carried out by our group. Key words: Vulvovaginitis, vaginitis, Candida spp. Candida albicans
2004

abstract No: 

none

Full conference title: 

14th Annual Focus on Fungal Infections
    • FFI 2004 (14th)