Abstract:
Aspergillus aculeatus secretes superior cellulolytic and hemicellulolytic enzymes on saccharification. It has been revealed that a Zn(II)2Cys6 transcription
factor, XlnR, positively regulated those gene expressions in Aspergillus. However, we found that the xlnR gene disruption did not affect the transcription
levels of cellobiohydrolase I (cbhI) and carboxymethylcellulase 2 (cmc2) genes in A. aculeatus. These data suggests that those genes are regulated by
namely XlnR-independent signaling pathway. To identify regulator(s) participating in the regulation network, we established a positive screening system
using a PcbhI-pyrG reporter fusion. Mutations were introduced by T-DNA insertion utilizing Agrobacterium tumefaciens-mediated transformation system,
and which enabled to rescue the franks of T-DNA by PCR-based technique. Transformants were selected by indexing 5-FOA resistance and cellulose
utilization deficiency, and then further analyzed on expression level of the cbhI and cmc2 by RT-PCR. One out of 6,000 transformants was, so far,
identified as a cellulase induction-deficient mutant. The T-DNA was verified to insert into near 5"¢f-end of ORF encoding putative a Zn(II)2Cys6
transcription factor, namely factor A. The factor A gene disruption by homologous recombination resulted in reduction of the cbhI and cmc2 transcripts,
demonstrating that Factor A participated in the XlnR-independent signaling pathway.
2011
abstract No:
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Full conference title:
26th Fungal Genetics Conference
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- Fungal Genetics Conference 26th (2005)