Mating-type (MAT) genes have been identified from the pyrenomycete, loculoascomycete and discomycete classes of ascomycete fungi. By using hot-start PCR with degenerate primers, together with thermal asymmetric interlaced (TAIL)-PCR, it has been possible to identify a characteristic MAT-2 gene from the plectomycete fungus Aspergillus nidulans. It includes a conserved high mobility group (HMG)-domain. RACE-PCR analysis has been used to investigate transcription of the gene, confirming the presence of an intron in a conserved position within MAT-2 genes. Further analysis of the flanking regions of the MAT-2 gene has revealed three further genes, including a putative gene with homology to an element of the anaphase-promoting complex (APC) of Schizosaccharomyces pombe. The presence of an APC homologue provides evidence of microsyteny around the MAT locus. However, no MAT-1alpha-domain gene could be detected. This suggests either that a MAT-1 homologue may be present elsewhere in the genome, not directly adjacent to the MAT-2 locus, or thatA. nidulans may contain only a MAT-2 mating-type gene. This would be a unique situation among homothallic euascomycetes so far analysed, which contain either aMAT-1 gene or both MAT-1 /MAT-2 genes.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)