Identification of Malassezia isolates of patients with pityriasis versicolor by using PCR-restriction enzyme method

H. Mirhendi, K. Makimura, B. Tarazooei, N. Jalalizand

Author address: 

Tehran, IR; Tokyo, JP


Introduction: The member of the genus Malassezia are the causative agents of the skin disease pityriasis versicolor. There are evidences that these yeast may involve in some other dermatological disorders such as seborrheic dermatitis and atopic dermatitis. The recently finding shows various Malassezia species can cause the diseases and molecular biological methods are the most reliable way for identification of the isolates. The aim of the present study is identification and determination of frequency of Malassezia isolates in Iranian patients with pityriasis versicolor by using PCR-restriction method. Methods: 83 patient's isolates of Malassezia were isolated on modified Dixon agar. Genomic DNA was extracted by glassbeads-phenol chloroform method and purified by alcohol precipitation. In all samples 28srDNA regions was PCR-amplified, digested by the restriction enzyme CfoI and electrophoresed on 1.8% agarose gels. Identification was carried out according the different electrophoretic patterns after RFLP. Results: The most frequent isolate was M. globosa (63.85%), followed by M. furfur (28.9%), M. sympodialis (3.61%), M. restricta (2.4%) and M. slloffiae (1.2%), respectively. Conclusion: 1. PCR-RFLP is an accurate, rapid and straight forward method for differentiation of Malassezia species. 2. The most frequent causative agent of pityriasis versicolor in Iran should be M. globosa.

abstract No: 


Full conference title: 

16th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 16th (2006)