Identification of Candida species Isolated from Hospitalized Patients with Candidemia in Tehran by Enzymatic digestion of ITS-rRNA

M. Ghahri1, H. Mirhendi2

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Background: Invasive candidiasis exists in various forms including candidemia, acute or chronic hematogenously disseminated candidiasis, and infection of a single deep organ, either by hematogenous seeding or direct inoculation. Candidemia is defiend as the isolation of Candida spp., from at least one blood culture specimen. Candidemia may or may not be accompanied by clinical signs of infection for example, fever, chills, or the sepsis syndrome. Candidemia may give rise to hematogenous spread of Candida to one or multiple organs. In Iran, many cases of candidemia remains undiagnosed, simply because of absence of adequate and/or serious attentions on it. Materials and Methods: A total of 48 blood positive cultures from 32 hospitalized, immunocompromized patients were provided. In addition of using API 20 AUX, Chromagar candida, Corn meal agar with tween 80 and Germ-tube test, a PCR-RFLP method were also used for identification of Candida spp. Boiling method were used for DNA extraction and ITS1-5.8s- ITS2 region were amplified. Msp1 and Mbo1 restriction enzymes were used for accurate identification of Candida at the species level. Results: Candida parapsilosis was the commonest yeast isolated from candidemic patients (33.3%) in this study. C. glabrata (30%), C. albicans (26.6%), C. tropicalis (6.7%) and C. kefyr (3.3%) were identified as another cause of candidemia in Tehran hospitals. 64.5% of isolates were identified as Non-albicans Candida species. Conclusion: In present study we were able to detect some significant cases of candidemia among hospitalized patients in Tehran hospitals. Innate or acquired resistant to antifungals in species such as C. glabrata needs more consideration. Also, the prevalence of C. parapsilosis in present study should be considered and it seems more studies is necessary to better evaluation of hospital quality services in Tehran. Keywords: systemic candidiasis, PCR identification, C. albicans

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Iranian Congress on Medical Mycology
    • ICMM 2011