IDENTIFICATION OF ASPERGILLUS FUMIGATUS AND RELATED VARIANTS BY NESTED PCR USING SPECIES-SPECIFIC PRIMERS

Jun Zhao, Fanrong Kong, Ruoyu Li, Xiaohong Wang, Zhe Wan, Duanli Wang

Abstract: 

A.fumigatus is the most common species that causes invasive aspergillosis (IA). In order to identify A.fumigatus, partial rDNA from 2 to 6 strains of 5 different Aspergillus species was sequenced. Compared to sequence data from GenBank, we designed species-specific primer pairs for A.fumigatus. A nested PCR method for identification of other Aspergillus species was established by using the primers To evaluate the specificity and sensitivity of those primers, 24 isolates of A.fumigatus and variants, 8 isolates of A.nidulans, 7 isolates of A.flavus and variants, 8 isolates of A.terreus, 9 isolates of A.niger, 1 isolate of each of A.parasificus, A.penieilloides, A.versicolor, A.wangduanlii, A.qizutongii, A.beijingsis, E.dermititidis, 9 isolates of Candidia, 9 isolates of bacteria and human DNA were used, respectively. The methods specifically identified A.fumigatus sect. and showed a high sensitivity. Additionally, four A.fumigatus strains, that were isolated recently from our clinic, were identified correctly by this method. These results demonstrate that the primers were useful for the identification of Aspergillus species in culture and suggest further studies to identify Aspergillus species in clinical specimens.
2001

abstract No: 

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Full conference title: 

Focus on Fungal Infections 11, March 14-16 2001
    • Focus on Fungal Infection 11 (2001)