HOW DOES DNA SEQUENCING FIT INTO A CLINICAL MYCOLOGY LABORATORY FOR THE IDENTIFICATION OF YEAST AND FILAMENTOUS MOLDS?

Hall L, Wohlfiel SL, Roberts GD

Author address: 

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Abstract: 

To determine how to use DNA sequencing in the clinical mycology laboratory, we evaluated the MicroSeq ® D2 rDNA Fungal Identification System (D2) (Applied Biosystems, Foster City, CA) in a routine clinical laboratory setting. The 500 16S rDNA Bacterial Identification System is used in our laboratory for the identification of mycobacteria and bacteria and has a 24-hour turn around time in our hands. D2 results in a similarity index (SI) and 0.0% is a considered a perfect match. Selected organisms recovered from patient specimens included yeasts that were difficult to characterize by API 20C AUX (20C) (bioMerieux, Durham, NC). These uncommon species (N=10) included Candida colliculosa (Torulaspora delbrueckii), C. rugosa, C. ciferrii (Stephanoascus ciferrii) (3), C. famata (Debaryomyces hansenii), C. utilis (Pichia jadinii), Pichia olmeri, Malassezia pachydermatidis and Trichosporon mucoides. D2 gave SI
2003

abstract No: 

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Full conference title: 

The 15 th Congress of the International Society for Human and Animal Mycology
    • ISHAM 15th (2003)