Rationale Genome sequencing of various fungi including Aspergillus fumigatus facilitate the transcriptomic profile of tissue of interest, therefore, we deduced the transcriptomic profile of A. fumigatus against host innate immune cells such as macrophage that may shed light on its complex biological behavior. Methods A. fumigatus specific oligo-arrays (Version I) from The Institute for Genomic Research, MD, USA, were used to obtain the transcriptomic profiles of A. fumigatus conidia incubated alone and with murine macrophage (J774A.1) in tissue culture flask containing RPMI media for 2.5 hours at 37 Â°C. Dye swap hybridization from two independent biological replicates was performed. Direct intensity-based normalization by median was used to normalize the data. Differentially expressed genes, either up or down 2 folds, were functionally classified based on yeast catalogue developed by the Munich Information Centre for Protein Sequences (MIPS). Results Up-regulation of signal transduction pathway (No of genes, N = 12), transcriptional regulation (29), carbohydrate metabolism (29), secondary metabolism (36), DNA/RNA metabolism (70), transportation (35), and hypothetical proteins (200) were observed. Proteins metabolism (52), carbon-6 metabolism (57), amino acid metabolism (18), urea metabolism (7), allergens (15), chromosomal packaging (4) and hypothetical proteins (69) were down-regulated. Real-time RT-PCR of selected genes such as Polyketide synthase type I (348 folds up) and Mono-oxygenase gene (3 folds up) Vs Actin as a housekeeping gene showed good correlation with microarray data. Conclusions Present data provides platform to study the molecular network during host-pathogen interactions that include signaling pathway, transcriptional regulation of gene, secondary metabolites, and also on several hypothetical proteins with unknown function.
Full conference title:
2008 American Academy of Allergy, Asthma, and Immunology Annual Meeting
- AAAAI 2008 (64th)