) Gliotoxin- exploiting its anti-fungal effects

Emer Molloy, Stephen Carberry, Markus Schrettl, Gary Jones, Karen O'Hanlon, Kevin Kavanagh and Sean Doyle.

Author address: 

Biology, NUI Maynooth, Co. Kildare, Ireland

Abstract: 

Aspergillus fumigatus biosynthesizes gliotoxin, yet little is known about the effect of gliotoxin on A. fumigatus. Exposure of A. fumigatus to gliotoxin (14 microg/ml) for 3 hours results in the de novo expression of Cu,Zn SOD, a short chain dehydrogenase and a eukaryotic translation elongation factor (beta1 subunit). Exposure also significantly altered the expression (up and down-regulated > 2x) of 27 other proteins including (i) upregulated: GliT (AFUA_6G09740; reported elsewhere), adenine phosphoribosyl transferase and allergen Asp f3 and (ii) down-regulated: catalase and a peroxiredoxin. Targeted deletion of gliT resulted in hypersensitivity of A. fumigatus delta gliT to gliotoxin (reported elsewhere). Re-introduction of gliT into A. fumigatus deltagliT, using a split marker approach, at a different locus (ctsD;AFUA_4G07040) with selection on gliotoxin-containing media, resulted in deletion of ctsD. Absence of ctsD expression was accompanied by restoration of gliT expression (RT-PCR), and resistance to gliotoxin. Significant (P A. nidulans, A. terreus, A. niger, Cochliobolus heterostrophus and Neurospora crassa was observed at 5 microg/ml gliotoxin. Growth of A. flavus, Fusarium graminarium and A. oryzae was significantly inhibited (P
2010

abstract No: 

41

Full conference title: 

7th International Aspergillus Meeting
    • Asperfest 7 (2010)