Thielavia terrestris (anamorph = Acremonium alabamense) is a thermophilic ascomycete that is of interest as a potential source of thermostable enzymes for biotechnological applications such as biomass decomposition. A high-quality draft genome sequence of T. terrestris NRRL 8126 was recently completed at the Joint Genome Institute. Subsequent mining, editing, and annotation efforts are in progress by an international team of collaborators. The genome assembly comprises eight scaffolds (231 contigs) spanning 36.9 Mbp (sequence coverage = 10.15x). The overall G+C content, excluding mitochondrial DNA, was approximately 58%. The presence of telomeric repeats [(TTAGGG)n/(CCCTAA)n] at both ends of scaffolds 1,3, 4 and 6, and at one end of scaffolds 2, 5, 7 and 8 suggests that the assembly contains nearly complete chromosome sequences. Among the 9815 predicted protein-coding genes in the Thielavia genome, >750 transposases were identified on the basis sequence identity with Aspergillus nidulans transposons, and a sizeable proportion of these appear to be degraded by RIP. Approximately 6-8% of the gene models are predicted to encode secreted proteins such as oxidoreductases, peptidases and a variety of glycoside hydrolases. Compared to the well-studied cellulolytic fungus Trichoderma reesei, an obvious expansion of genes encoding family GH61 proteins was noted. Nimblegen expression arrays were deployed in a preliminary investigation to compare the transcription profiles of T. terrestris cells grown on several substrates (e.g., glucose, cellulose, xylan, soy flour), and induction of genes predicted to encode cellulases and hemicellulases was observed on cellulose and xylan, respectively. A comparison of transcriptome data for cells grown in glucose medium at 34Â°C and 45Â°C suggested that growth of T. terrestris at the higher temperature may induce expression of genes encoding membrane proteins, sterol biosynthetic enzymes, heat shock proteins/chaperones and components of the ubiquitin proteasome pathway.
Full conference title:
10th EUROPEAN CONFERENCE ON FUNGAL GENETICS
- ECFG 10th (2010)