gcsA, an ARF-GAP-Encoding gene in A. fumigatus.

Ricardo Almeida , Ana Carolina Gratieri Maciel , Gustavo Goldman , Iran Malavazi, Marcia R. von Zeska Kress

Author address: 

Pharmaceutical Science Faculty of Ribeirao Preto, USP; 2 UEL-PR; 3 Genetics and Evolution Department, UFSCar, SP, Brazil ([email protected])


Aspergillus fumigatus is one of the most important airborne pathogenic fungi, which has the potential to cause Invasive Pulmonary Aspergillose (IPA). Sphingolipids are the major component of the eukaryotic plasma membrane and are involved in the cell wall integrity and virulence. The ADP-ribosilation factor (ARF) family of proteins belongs to the Ras superfamily of small GTPases. The hydrolysis of ARF GTP-bound is mediated by GTPase-activating proteins (GAPs). ARF-GAPs are required for vesicular coat formation in endocytic pathway and have been related to hyphal growth, drug resistance and virulence in C. albicans. In this work we identified gcsA, the C. albicans age3 ortholog gene in A. fumigatus. gcsA null mutant has normal hyphal growth, and decreased cell polarization in the presence of Myriocin, an inhibitor of serine palmitoyltransferase, the first step in sphingolipids biosysthesis. The absence of gcsA gene shows no differences in sensitivity to members of antifungal classes. The null mutant was not able to form biofilm, and was attenuated in virulence in an IPA mouse model. Thus, despite the gcsA null mutant has shown a normal sensitivity to antifungal agents, it has presented a role at the sphingolipids biosinthesis and virulence attenuation, suggesting a probable distinct function of this gene in A. fumigatus. Acknowledgments: FAPESP

abstract No: 


Full conference title: 

Asperfest 9
    • Asperfest 9 (2012)