Objectives: Last years there is an increasing trend of isolation of fungi from airway secretions of patients with cystic fibrosis (CF), which is accompanied with the presence of new fungal species. The aim of our study was to determine the prevalence as well as the species of fungi from airway secretions of children with CF. Material and Methods: During a 23 months period (February 2007 - December 2008) 2770 sputum or deep throat cultures from 393 children with CF (mean age 8.9 Â± 3.8 years) were performed. Sabouraud dextrose agar (Conda Pronandisa, Spain), with Gentamicin, was used for the culture of fungi, incubated at 35 oC for up to 5 days. The identification of filamentous fungi was based on the macroscopic and microscopic morphological features in accordance with standard descriptions. Identification of yeasts was based on the carbohydrate’s assimilation (API 20 AUX, Biomerieux). Results: 426 cultures from 221 children were positive for fungi (15.4 %). Multiply isolates of the same fungus per patient were included only once. The prevalence of potentially pathogenic fungi from patients with CF as Candida albicans, Aspergillus fumigatus, Scedosporium apiospermum and Exophialla (Wangiella) dermatitidis was 62.9, 11.8, 3.8, and 0.4 % respectively. All species of fungi isolated from the 221 children are presented in the table below: Conclusions: Suitable culture methods for the isolation and identification of fungi to species level is essential for patients with CF. The reason is that fungi participate in the inflammatory process and allergic bronchopulmonary reactions of these patients as well as that new pathogenic species are described, often resistant to many antifungal agents as Scedosporium apiospermum and Exophialla (Wangiella) dermatitidis.
Full conference title:
19th European Congress of Clinical Microbiology and Infectious Diseases
- ECCMID 19th (2009)