Fungemia during murine cryptococcosis sheds some light on pathophysiology

Lortholary, O.;Improvisi, L.;Nicolas, M.;Provost, F.;Dupont, B.;Dromer, F.

Author address: 

Dromer, F, Inst Pasteur, Unite Mycol, 25 Rue Dr Roux, F-75724 Paris 15, France


We studied fungemia over time in outbred mice infected with Cryptococcus neoformans and looked at its relationship with the intravenous (i.v,) inoculum size, tissue burden and survival. Fungemia was evaluated by culture of 10 mu l of peripheral blood from living mice or by culture of buffy coats from sacrificed animals. For all inoculum sizes studied, fungemia could last several weeks after the i.v. inoculation. Individual susceptibility of outbred mice to cryptococcal infection was evidenced by variations in the course, duration and magnitude of fungemia and tissue localizations. These results suggest that the fungus can recirculate after the initial i.v. inoculation. Fungemia, assessed by culture of buffy coats, correlated with the extent of infection in the spleen, lung or brain (P

abstract No: 


Full conference title: 

38th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 38th