Fungal Infections in Patients after Solid Organ Transplants

Irena Netsvyetayeva1, Ewa Swoboda-Kopec1, Dariusz Kawecki1, Magdalena Sikora1, Sylwia Blachnio, Magdalena Durlik, Andrzej Chmura, Leszek Paczek, Miroslaw Luczak.

Author address: 

1Dept. of Medical Microbiology Medical University of Warsaw, Poland; 2Dept. of Transplantation Medicine and Nephrology Medical University of Warsaw, Poland; 3Dept. of General Surgery and Transplantation Medical University of Warsaw, Poland; 4Dept

Abstract: 

Introduction: Fungal infection is a severe complication in patients undergoing solid organ transplantations. Objective: The objective of the study was to evaluate species distribution and antifungal susceptibilities of fungal isolates taken from patients after solid organ transplantation (SOT). Material and Methods: The study included samples of urine, blood, systemic fluids and the swabs of the post-operative wounds, haematomas, drains taken from patients after kidney, liver or simultaneous pancreas-kidney transplantation hospitalized in Institute of Transplantation Medicine, Medical University of Warsaw and Department of General and Transplantation Surgery, Medical University of Warsaw in 2005- 2007. All cultured specimens were isolated by using Sabouraud medium with antibacterial protection using chloramphenicol and gentamicin (bioMerieux, France or Oxoid, England). Yeast-like fungi was incubated on CHROMAgar Candida Medium (Becton Dickinson R) and identified by using biochemical, automatic test ID32C (bioMerieux). Susceptibility of the strains to the antifungal agents: amfotericin B, itraconazol, fluconazol, voriconazol was tested using E-test (AB Biodisk) on RPMI agar plates (BiolifeR). Results: The positive cultures were obtained in 662 samples from 402 patients receiving immunosuppressive therapy. There were cultured 267 isolates of Candida albicans, 223 Candida glabrata, 39 strains of C. parapsilosis, 37 C. krusei and 35 of Trichosporon asahii, 20 Saccharomyces cerevisiae, 9 strains of Cryptococcus neoformans 5 C. lipolytica, 6 strains of C. lusitaniae and 4 of C. sake, 5 strain of C. quilliermondii, 3 C. rugosa and 9 moulds from Aspergillus fumigatus species. From cultured strains 37.3% was naturally fluconazole resistant and 0.5% amphotericin B resistant. The acquired resistant to fluconazole was presented by 10 isolates, to itraconazole 26, to voriconazole 2 and to amphotericin B by 3 isolates. Conclusions: The most numerable groups of isolated fungal species in patients after SOT were C. albicans 40% of all isolates and C. glabrata 33.7%. Moulds were cultured less often and they were found only in 1.4% of positive cultures. More than one third of isolated fungi were resistant to fluconazole.
2008

abstract No: 

S21

Full conference title: 

15th International Symposium on Infections in the Immunocompromised Host
    • ISIIH 15th