Facile synthesis of silver nanoparticles using onion extract and evaluation of its anti candidal activity

Umamaheswari. K*.Abirami. M, Aruna Sharmili and Jagannathan G

Author address: 

Department of Biotechnology, University of Madras, Guindy, Chennai- 600 025


Nanoscale materials have emerged up as novel antimicrobial agents owing to their unique chemical and physical properties. The synthesis of silver nanoparticles involves physical, chemical and biological methods, while there is a growing need to develop ecofriendly and sustainable methods for the synthesis of nano materials that do not use toxic chemicals in the synthesis protocols so as to avoid adverse effects in medical applications. In the present study, we report the facile synthesis of silver nanoparticles and its anticandidal activity. Silver nanoparticles was synthesized by bio reduction of silver nitrate using onion extracts and exposed to sunlight for 15 minutes. The synthesized nanoparticles were characterized by UV visible spectroscopy and the size and shape of the nanoparticles were determined by HRTEM. The silver nanoparticles showed a characteristic peak at 417 nm by UV spectroscopy and the size of the particle ranged from 5-17nm and was spherical in shape. The inhibitory activities of the silver nanoparticles were tested on non albicans Candida species such as C. tropicalis, C.fermentati, C. krusei and C. guillermondii by micro broth dilution method and the MIC values were recorded. The nanoparticles showed good inhibition against all the tested species of Candida and the MIC values ranged from 39ng/ml to 53(xg/ml. The present study showed the facile and eco friendly synthesis of silver nanoparticles using onion extracts and results of the susceptibility testing suggest that silver nanoparticles could be used as potent alternative antifungal agent. ’Corresponding author E-mail: [email protected]

abstract No: 


Full conference title: 

Society for Indian Human and Animal Mycologists 2014
    • SIHAM