Fungi produce secondary metabolites which can be good candidates for bioactive agents. It is difficult, however, to obtain the metabolites from cell culture as fungi produce them only under certain particular conditions. If we could design culture condition under that desirable secondary metabolites are produced, such knowledge should lead to discovery of novel secondary metabolites. Toward this goal, we analyzed gene expression distribution of Aspergillus oryzae under more than 200 culture conditions using DNA microarray. We used A. oryzae as a model although it produces bare secondary metabolites. The culture conditions are mainly classified into three categories: 1. nutrition, 2. time, and 3. chemical addition such as antifungal agents. Each category has sub-classes. Combined with another technology of ours to predict fungal secondary metabolic genes, expression distribution of secondary metabolic genes under each condition was hierarchically clustered. Interestingly, the grouped cluster of gene expression distribution overlaps with the class of culture condition to some extent. The same tendency was observed when selecting the genes considered to concern fatty acid synthesis. This result will lead to the clue to the conditions under that fungi tend to produce more secondary metabolites.
Full conference title:
26th Fungal Genetics Conference
- Fungal Genetics Conference 26th (2005)