The alpha-amylase gene from A. oryzae is subject to CREA-mediated carbon catabolite repression both in the host fungus and when expressed in a transgenic A. nidulans. An expression cassette containing a portion of the A. nidulans creA gene in the reverse orientation with respect to the gpdh promoter was introduced into the alpha-amylase-producing A. nidulans. This resulted in a several-fold increase in the enzyme production. The level of alpha-amylase mRNA in the antisense transformants was elevated. The presence of both senseand antisense-creA-transcript was detected. The kinetics of enzyme accumulation in control and antisense-derepressed strains was studied under well controlled fermentation conditions.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)