L-Arabinose residues, in furanose form, are widely distributed in plant tissue heteropolysaccharides, such as arabinans, arabinogalactans and arabinoxylans. The presence of arabinose as side groups can restrict enzymatic hydrolysis of hemicellulose. The T. reesei beta-xylosidase has been shown to have alpha-L-arabinosidase activities against small synthetic model substrates (Margolles-Clark et al., 1996, Applied and Environmental Microbiology, Vol. 62, No. 10, p. 3840-3846). We describe the cloning and expression of the T. reesei beta-xylosidase gene in the host, Aspergillus oryzae. Broth samples were analyzed for activity and the results show activity on the substrate xylan and even higher activity on p-nitrophenyl-xylose when compared with a positive control.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)