Experimental dermatophytosis in guinea pigs: is voriconazole an option for treatment?

D.M. Saunte, N. Frimodt-Moller, E.L. Svejgaard, M. Haedersdal, M.C. Arendrup

Author address: 

Copenhagen, DK

Abstract: 

Objectives: Standard treatment of tinea capitis caused by Microsporum (M.) species has for many years been oral griseofulvin which, however, is no longer marketed in all countries. Voriconazole (VCZ) has been demonstrated to inhibit M. canis in vitro. The aim of this study was to investigate the efficacy of voriconazole in a guinea pig model for dermatophytosis. Methods: Sixteen female Harlan (HsdSsc:AL) guinea pigs (n = 8/group) were inoculated with 5 x 105 M. canis cells/ml on razed skin. The VCZ-group was dosed p.o. 20 mg VCZ/kg/day in 12 days (day 3-15). The control group was left untreated. The guinea pigs were evaluated clinically twice a week and mycologically once a week. The clinical evaluation consisted of a redness score (0 = normal, 1 = pink, 2 = red, and 3 = violet) and a lesion score (normal = 0, papule = 1, scales = 2, scales (thin) and ulcers = 3, and scales (thick) and ulcers = 4). The mycological examination consisted of direct microscopy and culture of skin scrapings using Sabouraud-glucose-agar with chloramphenicol & cycloheximid incubated at 25ordm; C for four weeks. Species identification was performed based on micro- and macromorphology and the number of colonies was noted. Results: All animals had proven mycological infection. The VZC-treated group had significantly lower redness and lesion scores as compared to the control group (P = 0.03 (Mann-Whitney)). After 12 days of treatment 8/8 in the VCZ-treated group was microscopy negative and 7/8 culture negative in contrast to the control group where 8/8 animals were microscopy and culture positive and with significantly higher colony counts (mean colony count:
2006

abstract No: 

P1149

Full conference title: 

16th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 16th (2006)