Objective: The VITEK 2 System provides rapid, automated identification and susceptibility testing of yeast isolates. With the increasing prevalence of antifungal resistance, the ability to quickly and easily perform susceptibility testing with available antifungals is becoming more important. The purpose of this study was to determine whether caspofungin (CAS), micafungin (MCF) and posaconazole (POS) could be incorporated into the current VITEK 2 system menu for automated susceptibility testing of yeast. Methods: Over 450 isolates were tested in VITEK 2 IUO cards containing varying concentrations of CAS, MCF and POS. All of these strains were either Candida species or Stephanoascus ciferii. All strains were tested with all three antifungals using both IUO cards and a reference method. The reference method for all three was broth microdilution, which was performed according to Clinical Laboratory Standards Institute (CLSI) standards. Growth data were collected from the VITEK 2 cards and compared to the reference results. Analyses were then developed using these data. Development Results: Overall category agreement (CA) for CAS was 98.9%(449/454). Overall essential agreement (EA) was 99.6% (452/454). Overall category agreement (CA) for MCF was 99.6%(452/454). Overall essential agreement (EA) was 98.7% (448/454). Overall category agreement (CA) for POS was 95.4%(433/454). Overall essential agreement (EA) was 94.9% (431/454). Conclusion: These development data indicate that the VITEK 2 can accurately detect resistance to CAS, MCF and POS in Candida species and in Stephanoascus ciferii .
Full conference title:
19th European Congress of Clinical Microbiology and Infectious Diseases
- ECCMID 19th (2009)