Evaluation of the Sensititre Yo-9 Plate for Antifungal Drug Susceptibility Testing of Molds

R. C. WALCHAK, A. Abbenyi, A. A. Milone, N. L. Wengenack

Author address: 

Mayo Clinic Rochester, Rochester, MN

Abstract: 

Background: The performance of the commercially available Sensititre YeastOne YO-9 plate (ThermoFisher/Trek Diagnostics) was compared with the broth dilution reference method (CLSI M38-A) performed at a reference laboratory in order to evaluate the potential utility of the YO-9 plate for mold antifungal susceptibility testing.Methods: Well-characterized clinical isolates of molds including Aspergillus spp. and Fusarium spp. were tested in parallel using both the reference method and the YO-9 plate against 9 antifungal drugs including several azoles, amphotericin B, and three echinocandins. The MIC or MEC for each drug were determined by following the guidelines in the CLSI M38-A document.Results: The agreement between the reference laboratory and the YO-9 plate was 98.9% using an essential agreement acceptance range of +/- 2 doubling dilutions. There was a single discordant fluconazole result for a Fusarium sp. isolate.Conclusions: The Sensititre YeastOne plate compared favorably with the reference method in terms of essential agreement for each drug tested across a variety of mold genera and species. Using the Sensititre YeastOne plates also provides a simplified workflow compared with the reference method for implementation into a clinical laboratory.
2016

abstract No: 

FRIDAY-308

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)